Journal: PLoS ONE
Article Title: Post-weaning epiphysiolysis causes distal femur dysplasia and foreshortened hindlimbs in fetuin-A-deficient mice
doi: 10.1371/journal.pone.0187030
Figure Lengend Snippet: Decalcified paraffin sections were stained with antibodies for STAT1 and pSTAT1 (red) and nuclei were counterstained with DAPI (blue). The figure shows representative micrographs for each time point and each genotype recorded with a 20-fold (uppercase letter) and a 63-fold lens (lowercase letter). STAT1 and pSTAT1 signal was mainly localized in the hypertrophic zone. The signal was increased in Ahsg +/- and Ahsg -/- mice of all ages. Scale bars are 150 μm for micrographs taken at lower and 20 μm for micrographs taken at a higher magnification.
Article Snippet: The following primary antibodies were used for immunofluorescent staining: rabbit anti-mouse fetuin-A antiserum developed in-house [ ], polyclonal rabbit anti-mouse STAT1 IgG (#sc-346, Santa Cruz), monoclonal rabbit anti-mouse pSTAT1 IgG (#7649, Cell Signaling), polyclonal rabbit anti-mouse CD3 (#A0452, Dako).
Techniques: Staining